Last edited by Arakinos
Thursday, August 6, 2020 | History

2 edition of homogenous enzyme immunoassay for the measurement of methotrexate in human plasma. found in the catalog.

homogenous enzyme immunoassay for the measurement of methotrexate in human plasma.

Jeffrey Alan Cox

homogenous enzyme immunoassay for the measurement of methotrexate in human plasma.

by Jeffrey Alan Cox

  • 372 Want to read
  • 29 Currently reading

Published in Bradford .
Written in English


Edition Notes

M.Pharm. dissertation. Typescript.

SeriesDissertations
The Physical Object
Pagination42p.
Number of Pages42
ID Numbers
Open LibraryOL13728204M

  Assignment on General principles of Immunoassay 1. Immune& Assay Immuno refers to an immune response that causes the body to generate antibodies. Assay refers to a test. Thus immunoassay . A competitive protein binding assay has been developed for methotrexate based on the tight binding of this drug to Lactobacillus casei dihydrofolate reductase (= tetrahydrofolate dehydrogenase; 5,6,7,8 Cited by:

The immunoassay technology commonly used in this field is described: radioimmunoassay [not used much now], enzyme-multiplied immunoassay technique, cloned enzyme donor immunoassay, enzyme-linked immunosorbent assay, homogeneous fluorescence enhancement and quenching assays, fluorescence polarization and microparticle enzyme immunoassay. Immunoassay (enzyme linked immunosorbent assay, ELISA, or enzyme immunoassay, EIA)—the results are usually reported as a number with an arbitrary unit of measure (abbreviated as a "U" on .

This homogeneous thyroxine enzyme immunoassay is intended to be used for quantitative determination of total thyroxine in human serum or plasma. Summary and Explanation of the Test . Jeffrey Alan Cox has written: 'A homogenous enzyme immunoassay for the measurement of methotrexate in human plasma' Asked in Authors, Poets, and Playwrights What has the author .


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Homogenous enzyme immunoassay for the measurement of methotrexate in human plasma by Jeffrey Alan Cox Download PDF EPUB FB2

The ARK Methotrexate Assay is a homogeneous enzyme immunoassay intended for the quantitative determination of methotrexate in human serum or plasma on automated clinical chemistry analyzers. The Methotrexate assay is a homogenous, enzyme immunoassay, for the quantitative determination of methotrexate in human serum or plasma on automated clinical chemistry analyzers.

The ARK Methotrexate Assay is a homogeneous enzyme i]Immunoassay intended for the quantitative determination of methotrexate in human serum or plasma on automated clinical chemistry analyzers.

The measurements obtained are used in monitoring levels of methotrexate to help ensure appropriate therapy. Intended Use. The ARK Methotrexate Assay is a homogeneous enzyme immunoassay intended for the quantitative determination of methotrexate in human serum or plasma on automated clinical chemistry analyzers.

The measurements obtained are used in monitoring levels of methotrexate to help ensure appropriate therapy. Yalow, R.S. and Berson, S.A. () Assay of plasma insulin in human subjects by immunological methods. Nature, –9. PubMed CrossRef Google ScholarCited by: 6. 2 INTENDED USE.

The ARK Methotrexate Assay is a homogeneous enzyme immunoassay intended for the quantitative determination of methotrexate in human serum or plasma on automated clinical chemistry analyzers. The measurements obtained are used in monitoring levels of methotrexate to help ensure appropriate therapy.

Nanopia ® e TDM Methotrexate Intended use: quantitative determination of methotrexate in human serum or plasma Assay Principle: homogeneous enzyme immunoassay Measurement Range: μmol/L Sample Types: Serum, Plasma.

The enzyme-multiplied immunoassay technique (EMIT ®) is a simple, rapid homogeneous method now commonly employed to measure a wide range of substances (particularly drugs). The technique works on the basis that the drug present is proportional to the inhibition of an enzyme substrate reaction.

Methotrexate (MTX) plasma concentration is routinely monitored to guide the dosage regimen of rescue drugs. This study aims to develop and validate an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for plasma MTX analysis, and to establish its agreement with the fluorescence polarization immunoassay Cited by: 5.

and predominantly used technique for the measurement of MTX level in human plasma. [ 16] The majority of the reported HPLC assays used relatively large plasma volumes (up to 1.

The methotrexate assay is also appropriate for the detection of methotrexate contamination after its use as a selective agent for recombinant protein production in mammalian cell lines.

The ultrasensitive assay allows for detection of as little as ng/mL of methotrexate in human serum and plasma. Rapid determination of methotrexate level in human plasma by high performance liquid chromatography. Article (PDF Available) in WORLD JOURNAL OF PHARMACY AND.

Another approach to heterogeneous enzyme immunoassay is the microparticle enzyme immunoassay (MEIA). In this assay, antibodies directed toward the analyte of interest are immobilized to the surface of microparticles. AccessGUDID - ARK™ Methotrexate Assay ()- The ARK Methotrexate Assay c-Pack is a homogeneous enzyme immunoassay intended for the quantitative determination of.

Ngo and H. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA InYalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma.

Methotrexate was determined by the homogeneous enzyme immunoassay (EMIT) with the Multistat and CentrifiChem centrifugal analyzers and by the enzyme inhibition assay with use of the Multistat centrifugal by: 3.

United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK homogeneous enzyme immunoassay, PR QMC NCH Serum or Plasma Chloride Beckman AU, AU Serum or Plasma Methotrexate Beckman AU, AU Analyser, Homogeneous immunoassay.

The ARK™ Methotrexate Assay is a homogeneous enzyme immunoassay intended for the quantitative determination of methotrexate in human serum or plasma on automated clinical chemistry analyzers. The measurements obtained are used in monitoring levels of methotrexate.

AKR™ Methotrexate Assay (Display) The AKR™ Methotrexate Assay is intended for the quantitative determination of methotrexate in human serum or plasma on automated clinical chemistry analyzers.

The measurements obtained are used in monitoring levels of methotrexate to help ensure appropriate therapy. Key Points - Homogeneous enzyme Immunoassay. Large quantities of colorimetric assays for detecting tumor markers are proposed based on enzyme-linked immunosorbent assay (ELISA) [52–59].Conventional ELISA-based colorimetric immunoassays Cited by:.

The ARK™ Levetiracetam Assay is a homogeneous enzyme immunoassay intended for the quantitative determination of levetiracetam in human serum or plasma on automated clinical chemistry analyzers. The measurements .issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK The Christie Pathology Partnership LLP Issue No: Issue date: 22 April Testing performed at main address only Assessment Manager: GS2 Page 3 of 24 Materials/Products tested Type of test/Properties measured/Range of measurement.ARK™ Voriconazole II Assay The ARK™ Voriconazole II Assay is a highly specific homogeneous enzyme immunoassay for the therapeutic drug monitoring of Voriconazole in human serum or plasma.

The assay .